ecific endonucleases: implications or nucleotide excision repair

نویسندگان

  • John J. Harrington
  • Michael R. Lieber
چکیده

Structure-specific nucleases catalyze critical reactions in DNA replication, recombination, and repair. Recently, a structure-specific endonuclease, FEN-l, has been purified and shown to cleave DNA flap structures. Here, we describe the cloning of the murine FEN-1 gene. The nucleotide sequence of FEN-I is highly homologous to the 8accharomyces cerevisiae genes YKL510 and RAD2. We show that YKLS10 and a truncated RAD2 protein are also structure-specific endonucleases. The substrate specificity of the truncated RAD2 protein implicates branched DNA structures as important intermediates in nucleotide excision repair. The polarity of these branched DNA structures allows us to predict the placement of DNA scissions by RAD2 and RAD1/RADI0 in this reaction.

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تاریخ انتشار 2007